Department of Biomedical-Chemical Engineering, The Catholic University of Korea, 43 Jibong-ro Wonmi-gu, Bucheon-si, Gyeonggi-do, 14662, Republic of Korea, LG electronics, 19 Yangjae-daero 11-gil, Seocho-gu, Seoul, 06772, Republic of Korea, You can also search for this author in Structural and biocompatibility properties of dextran from The exponent a is a function of polymer geometry, and varies from 0.5 to 2 and exponent is dimensionless. Learn More. Superior prebiotic and physicochemical properties of novel dextran from Weissella cibaria JAG8 for potential food applications. Dextran Specifications: Label (Ex/Em): Fluorescein (494/521) Size: 40,000 MW. CAS Thomas Gutberlet (Lipoteichoic Acid): Statistiques d'influence Carbohydrate Research, 160, 205-214. WebThomas Gutberlet works at Jlich Centre for Neutron Science (JCNS-2), Forsch and is well known for Lipoteichoic Acid, Neutron Reflectometry and Neutron Reflectivity. The Solomon-Ciuta single-point method is used for calculating dextran intrinsic viscosity and the error relative is calculated, and %RE respect to Huggins method is >4.18% for 2000kDa, and >2.06% for 8.8kDa (Table 2). Home > 2022 > Junho > 22 > Uncategorized > dextran hydrodynamic radius. Dilute solution properties and degree of chain branching The value of kM-H in this work decreases with increasing temperature in contrast to published by reference [34] where kM-H increases. Palmuti Braga Vettoria, Mary Helen; Martins Franchetti, Sandra Mara; Contiero, Jonas (2012). The hydrodynamic 57 radius of the dextranase is 7 7 8 Ioan, C.E. The skin poration was performed using the microporation device (LG electronics, Korea). and transmitted securely. Pharm Res. Bookshelf (2003). Immediately after 2, 5, and 10 times microporations, 97.1432.39, 80.5715.51, and 82.3916.49m sizes of micropores were formed on the skin, respectively. Equivalent Radii and Ratios of Radii from Solution Properties as Indicators of Macromolecular Conformation, Shape, and Flexibility. dextran hydrodynamic radius 2013, Article ID 360239, pp. Dependence on Concentration. Mark-Houwink-Kuhn-Sakurada Exponent at the Condition. Biophysical Journal, 87, 4259-4270. WebTry out PMC Labs and tell us what you think. Sintov AC, Krymberk I, Daniel D, Hannan T, Sohn Z, Levin G. Radiofrequency-driven skin microchanneling as a new way for electrically assisted transdermal delivery of hydrophilic drugs. Results and Discussion The microscopic dynamics of proteins are probed in a light scattering experiment. 2011;5(2):95132. An official website of the United States government. In order to understand the behavior of this biopolymer in water and to support changes at the M-H parameters with temperature, the hydrodynamic parameters are analyzed (hydrodynamic radius, (RH); Simha number, ((a/b)); Perrin parameter (P); Flory parameter (0); and stiffness chain (dln[]/dT)). Pavlov, G.M. (2013). 2004;25(9):155967. Ficoll and dextran vs. globular proteins as probes for testing Takara, Andres; Acosta, Adolfo; Masuelli, Martin A. Therefore, the trend of "a" and kM-H decreases with T, where the presence of ethylene glycol or dimethylsulfoxide strongly changes the interactions of this solvent with the macromolecule. Epub 2012 Oct 18. Polymers | Free Full-Text | Confinement-Induced Fractionation and Dextrans in Aqueous Solution. 1984;17(6):11703. Interaction Parameter (); Expansion Factor (); Steric Hindrance Factor (); and Shielding Function F(); for the System PEAOrganic Solvents by Intrinsic Viscosity Measurements. Ortega, A.; Garca de la Torre, J. Intrinsic viscosity molecular weight relationship. These results could be attributed to the differences in the in vivo and in vitro skin nutrients supply; with in vivo studies, the skin tissue is supplied nutrients directly from the blood vessels, but the human cadaver skin is supplied from the media, therefore, the skin tissue regeneration efficiency is low [26,27,28]. At 20C data "a" value obtained in this work is intermediate to those given by references [1, 37] while the value of kM-H is lower. 24h after microporation, the pore sizes reduced from 64.8817.20m (0h) to 48.854.27m (24h), and the pore depths reduced from 83.9120.56m (0h) to 63.9527.61m (24h). 43-1, 115-147. MeSH terms Chem. dextran eCollection 2015. Named after George Gabriel Stokes, it is closely related to solute mobility, factoring in not only size but also solvent effects.A smaller ion with stronger hydration, for example, may have a greater Stokes radius than a larger ion with Structural characterization of a new dextran with a low degree of branching produced by Leuconostoc mesenteroides FT045B dextransucrase. However, the results did not show significant regeneration of micropore compared to previous in vivo studies which reported significant regeneration [25]. In a poor solvent which shows a positive heat of mixing, segments of a polymer molecule attract each other in solution more strongly than attract the surrounding solvent molecules. Carbohydrate Polymers, 78, 902-907. For data requests, please contact the authors. 4K and M.W. dextran Int J Pharm Investig. Structure Properties of Dextran. (1991). In addition, we evaluated the regeneration of SC after microporation. WebIn this work, hydrodynamic properties are investigated in different dextran aqueous solutions with different molecular weights (8.8-2000kDa), in a temperature range from 20C to 50C. In a good solvent, a temperature increase results in an intrinsic viscosity decrease and in a less-extended conformation (RH<), because the entropy value increases with an increase in temperature and it is unfavorable for an extended conformation. Many works are aimed at determining molecular weights [1, 7, 26], hydrodynamic radius, among other parameters for characterizing the physics and chemistry properties of dextran in solution. Cytotoxicity studies on human cervical cancer (HeLa) cell line showed non-toxic and biocompatible nature of dextran. what will you do to keep amazon safe answers; amarsi assisted living; peter clarke, emilia clarke; personality characteristics of kiran mazumdar shaw; karina mitchell age; mirela romanian orphan died; dsa presidential endorsement; dextran hydrodynamic radius. These vibrations heat the skin tissue, triggering water vapor and cell ablation, forming microchannels from the SC through to the outer dermis. (1954). It is worth noting that the size of macromolecules is much bigger than that of solvent (water) molecules [50]. Data File Dextran. US20230040324A1 AMINOACRIDINE AND AMINOPYRENE DYES Ann Surg Oncol. Dilute Solution. Treatment of acne scars by fractional bipolar radiofrequency energy. Determination of the intrinsic viscosity has been the most used measurement in the last 60 years. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. These results suggest that fibroblasts proliferate and migrate under the SC, and regenerate the skin. Dextran, Tetramethylrhodamine, 2,000,000 MW, Lysine In this work, the human cadaver skin was treated to confirm the skin regeneration after the application of the microporation device. As intrinsic viscosity of dextrans decreases with an increase in solution temperature; chain flexibility enhances with an increase in temperature (see Table 4) showing that the intrinsic viscosity of dextran (with different molecular weights Mw from 8.8 to 200kDa) decreases linearly with an temperature increase in solution, but change to high molecular weight to hyperbranched macromolecule. We will also conduct further studies on the regeneration of human cadaver skin and evaluate the possibilities of clinical trials. The genus Weissella: taxonomy, ecology and biotechnological potential. In the range of working temperatures and molecular weights of dextrans, similar intrinsic viscosity results obtained by other researchers and published here are slight, as shown in tables 1 and 3. The optical image and histological analysis confirmed that the micropores on the skin were recovered over time. On the molecular weight distribution of dextran T-500. H&E stained images were analyzed using Image J software. Gekko, K. (1971). 2012;29(7):201729. Its Invariancy with Respect to the Cross-Sectional Dimensions of Polymer Chains. De Belder, A.N. Table 2 compares the properties of nanoparticles coated with one or other polymer. High Manufacturing Standards of Molecular Probes Dextrans. The value of qM-H varies from one sample to another one, because it is a function of a and the average molecular weights (Mv, Mw). Biological skin substitutes for wound cover and closure. The receptor phase was collected immediately (time 0) and 2, 4, 8, 12, and 24h. This receptor phase solution was transferred to a 96-well plate and the skin-permeated FITC-dextran was measured using a microplate reader (Molecular Devices, Co. Ltd., Sunnyvale, CA, USA) at 405nm. 2012;3(1):208. Synthesis of water-soluble and water-insoluble amphiphilic derivatives of dextran in organic medium. Jeanes, A.; Haynes, W.C.; C. Wilham, A.; Rankin, J.C; Melvin, E.H.; Austin, M. J.; Cluskey, J. E.; Fisher, B.E. 2008;26(11):12618. ; Patel, T.R. (1954). Biodegradable dextran hydrogels crosslinked by stereocomplex formation for the controlled release of pharmaceutical proteins. WebThis value is sensible given that i j = 0 and that the search radius of the clustering algorithm is slightly larger than the average segment length of our Dextran model (b 5.5 nm). Progress in Measurement, Interpretation and Application to Structure in Dilute Solution. Chem. Beer, M.U. Various average molecular weights (Mv, Mn, Mw, and Mz) instead of Mv were substituted in M-H equation. RBECs were passaged onto cell culture inserts as described and cultured for a further 714 days in vitro. Increased epidermal laser fluence through simultaneous ultrasonic microporation. For a rigid or rod like polymer molecule that is greatly extended in solution, the Mark-Houwink a constant approaches to a value of 2.0 [48].The hydrodynamic radius (RH), for a sphere ((a/b) = 2.5) is given by the Einstein relation [49]. The column was eluted with bidestilled water a flow rate of 0.8 ml/min, injection 5L, back pressure 200psi. Schematic illustration of transdermal delivery after the application of the microporation device. The study of biopolymers as dextran in aqueous solution, is effectively determined by intrinsic viscosity [] measurements at different temperatures. Boudry I, Blanck O, Cruz C, Blanck M, Vallet V, Bazire A, Capt A, Josse D, Lallement G. Percutaneous penetration and absorption of parathion using human and pig skin models in vitro and human skin grafted onto nude mouse skin model in vivo. dextran hydrodynamic radius Dextrans in Aqueous Solution. Experimental Review on Intrinsic Chapter 2.1.4 Suspended level viscometers for transparent liquid, Ubbelohde Viscometer page 28-31. Dextrans can function efficiently as anterograde or retrograde tracers, If there are particles in the sample, the incident laser light gets scattered in all directions. Biomaterials Research Unperturbed dimensions and the theta temperature of dextran in ethylene glycol solutions. (2007). Lucifer yellow (LY) and FITC-labelled dextran stock solutions were prepared in Ringers-HEPES buffer (150 mM NaCl, 3.4 mM CaCl 2, 1.2 mM MgCl 2, 5.2 mM KCl, 0.5 mM NaHCO 3, 2.8 mM glucose, and 10 mM HEPES) and frozen at 20C. The importance of this type of study lies in the analysis of the polysaccharide behavior at industrial processes, the requirements to reduce energy, avoid flow problems and product quality control. Solvent effects on polysaccharide conformation. In summary, we demonstrated effective enhancement of skin permeability and delivery of macromolecules lower than 20KM.W., and regeneration of human cadaver skin after microporation with an RF-based microporation device. There is a deviation from linearity after 580kDa [69, 70]. Guner, Ali (1999). Keywords: intrinsic viscosity, dextrans, Mark-Houwink parameters, hydrodynamic, temperature, Journal of Polymer and Biopolymer Physics Chemistry, 2013 1 (1), Sachdeva V, Banga AK. Transdermal drug delivery: overcoming the skins barrier function. Transdermal drug delivery system for non-steroidal anti inflammatory drugs: a review. Alternatively, the value of qM-H can be calculated using numerical methods and other-average molecular weights (Mn, Mw, Mz) according to where c and d are empirical polynomial functions of the exponent a. Anal. Dextran is a branched polysaccharide, composed of -D-glucopyranosyl residues. Journal of Applied Polymer Science, 27, 795-810. dextran Tirtaatmadja, V.; Dunstan, D. E.; Boger, D. V. (2001). The Hydrodynamic Radii of Macromolecules and Their Effect on Red Blood Cell Aggregation. Please enable it to take advantage of the complete set of features! The mostly used commercial product/ polysaccharide is dextran, and it is produced from bacterium Leuconostoc mesenteroides, with an overall degree of branching of about 5% [1-3][1]. Increased TEWL values are evidence of micropores formation. 8580 Macromolecules 34, Notes - NIST Actually, everything depends on solvent you want to use and temperature. If you want to know gyration (Rg)or hydrodynamic (Rh) radius, you should Modelling the endothelial blood-CNS barriers: a method for the Progress in Biophysical Molecules Biological, 68, 207-262. PMC Thermo Fisher Scientific. Naik A, Kalia YN, Guy RH. The receptor chamber was filled with PBS. A single frequency laser is directed to the sample contained in a cuvette. (1958). (2001). Figure3 shows a magnified image of human cadaver skin with microchannels formed by the microporation device (250kHz) and the skin closure over time. Int J Food Sci Nutr. MeSH When applied to adherent cell cultures, the APGD-t is capable of inducing temporary cell permeabilization. The basic setup of a DLS instrument is shown in Figure 1. (1998). Solution properties of branched dextran. One day before the experiment, the skin was stabilized in the skin culture medium. A solvent at this temperature is called a theta solvent [53]. The cumulative permeation of FITC-dextran 10K was 4.820.32M, in the human skin and 5.920.46M in the pig skin. 2023 BioMed Central Ltd unless otherwise stated. We offer more than 50 fluorescent and biotinylated dextran conjugates in several molecular weight ranges. Dextran (DX) is one type of EPS synthesized by LAB in the presence of sucrose (R g), radius of hydrodynamic (R h), polymer conformation parameter ( = R g /R h), intrinsic viscosity ([]), Mark-Houwink coefficient () and branching degree of DXs of RPYs prepared under different conditions. Disclaimer. The skins were from the abdomen of a 39-year-old caucasian woman (BMI: 24). WebThe hydrodynamic radius ( Rh) determined by our Fluidity instruments is the Stokes radius, or the size of a hard spherical particle that diffuses at the same rate as the protein (s) detected. Measurements were made with a Ubbelohde C type suspended level viscometer [57] (IVA, Argentina); with a diameter capillary of 0.81mm and water draining time of 35.89s at 20C, with viscometer constant of 0.027883 mm2/s [58]. Carbohydrate Polymers, 79, 380-390. 8600 Rockville Pike These structural differences can significantly affect FITC-dextran delivery due to even after microporation [30]. Arch Dermatol Res. 2003;10:33847. Hennink, W.E. Middelkoop E, Bogaerdt AJ, Lamme EN, Hoekstra MJ, Brandsma K, Ulrich MMW. Table1 shows TEWL values measured immediately after the microporation of human and pig skins. Intrinsic viscosity of bovine serum albumin conformers. It has been found that, when applied to water treatment, it can effectively act on refractory organic matter in wastewater through thermal decomposition and water molecular action to produce free Barrier integrity was examined using Tewameter to confirm the poration of human cadaver skin using a microporation device. Covis, Rudy; Ladaviere, Catherine; Desbrieres, Jacques; Marie, Emmanuelle; Durand, Alain. The mechanism of the dextran-induced red blood cell Aggregation. TEWL measurements were performed before and 30min after microporation. https://doi.org/10.1186/s40824-020-00201-7, DOI: https://doi.org/10.1186/s40824-020-00201-7. Unable to load your collection due to an error, Unable to load your delegates due to an error. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. WebThe micelles produced at pH 4.6 have a spherical shape and their size is dependent on the Maillard reaction: reaction time, molar ratio of casein to dextran, and molecular weight of dextran used. WebDiffusivity of 2000 kDa Dextran was significantly lower for both hydrogel formulations, but was insensitive to collagen concentration, likely due to a larger hydrodynamic diameter (d h ~ 70 nm) on order of the collagen mesh size. Table 5 shows the data of Mark-Houwink parameters determined in figure 4 from linear regressions with 2 values higher than 0.9797. Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Thomas Gutberlet (Lipoteichoic Acid): Statistiques d'influence Microporation techniques for enhanced delivery of therapeutic agents. Hi Sir Alexander Malm , Thank you very much! Really appreciate your help! The a value is higher than calculated in this work, because these calculations were performed with number average molecular weight (Mn) which rearranges the value of a and forces him to enter into unbranched or linear molecules (a > 0.5), on the other hand, if molecular weights (Mw) ware used to value would be less than 0.5. The permeability of fluorescein isothiocyanate-dextrans (FITC-dextrans) with different molecular weights through the pig and human cadaver skins were measured using Franz diffusion cell. Microporation applications for enhancing drug delivery. (2003). Dextran In the case of a poor solvent, a temperature increase causes an increase in entropy and intrinsic viscosity and is favorable for the extended conformation. Journal Non-Newtonian Fluid Mech., 97, 295-301. After 8h, the micropore became more distorted from the previous circle, suggesting that the micropores were closing. dextran hydrodynamic radius Human cadaver skins (fresh human full-thickness skin disc, 12~20mm) were supplied by Biopredic International (Saint-Grgoire, France). Transdermal drug delivery is an attractive alternative that addresses the limitations of oral and parenteral routes of drug administration; it enables controlled release and long-term systemic drug delivery through the skin, avoids hepatic first-pass effect, avoids gastrointestinal drug degradation, reduces discomfort and trauma resulting from hypodermic injections, and prevents safety hazards associated with dangerous medical waste from needles [1, 2]. For 40 kDa molecules, the hydrodynamic diameter d h ~8 nm is much smaller than the collagen mesh size [33,41]. 2. IV. Immediately after formation, the microchannels are filled with interstitial fluid, making them hydrophilic, and a suitable drug delivery system [15]. The value of Mv is different from Mn, Mw, and Mz in a polydisperse polymer sample [38, 60, 61, 62]. American Journal of Food Science and Technology 2013, 1(3), 60-66. DLS tests were conducted at detection angle of 90 (standard) and 173 (backscatter) to measure protein hydrodynamic radius, while protein charge was examined in phase analysis light scattering (PALS) mode at detection angle of 15 (forward). Synthesis and characterization of blue dextrans. Journal of American Chemical Society, 64(11), 2716-2718. Enhanced topical delivery of small hydrophilic or lipophilic active agents and epidermal growth factor by fractional radiofrequency microporation. Carbohydrate Polymers, 36, 319-326. The polymer molecule assumes a tighter configuration, and the solution has a lower intrinsic viscosity. official website and that any information you provide is encrypted TEWL measurements were taken 30min after microporation of the human and pig skin. ; Rani, K.Y. Charge: Anionic. The cumulative permeation of FITC-dextran 10 K was 4.82 0.32 M, in the human skin and 5.92 0.46 M in the pig skin. Exp Cell Res. Molecular weight, particle size and diffusion coefficient can be determined in many studies by Dynamic Light Scattering [20, 21, 22], Gel Permeation Chromatography [23], Analytical Ultracentrifuge [24, intrinsic viscosity [1, 7, 14], size exclusion chromatography / multiangle laser light scattering / differential viscometry, Flow Field-Flow Fractionation [25], and so on. The equation 4 can be rearranged and resulted in a modified M-H equation as follows: where the value qM-H is the polydispersity correction factor, and it is a statistical function of the molecular weight distribution. Drug Deliv. ; Veldhuis, T.F.J. The value of P increases from 1.0272 to 1.2694 (spherical shape with tendency to spheroid for high Mw). WebR H Hydrodynamic radius [m] The basic DLS setup. Regulating dynamic signaling between hematopoietic stem cells Journal of Applied Polymer Science, 72, 871-876. Table 3 shows the intrinsic viscosity for the rest of the dextrans obtained by using the single-point method, measured at different temperatures. Viscosity of Liquids. For simplicity, macromolecules are treated as rigid molecules for a hydrodynamic study. Granath, K. A. par | Juil 2, 2022 | mitchell wesley carlson charged | justin strauss net worth | Juil 2, 2022 | mitchell wesley carlson charged | justin strauss net worth 113, 81-86. PubMedGoogle Scholar. FOIA Kumar SV, Tarun P, Kumar TA. 2019;26(1):25261. Bohdanecky, Miloslav (1996). Appl Biochem Biotechnol. Pig skins (Micropig Franz cell membrane) were supplied by Medi Kinetics Co., Ltd. (Pyeongtaek, Korea). The 70-kDa FITC dextran reached the jejunum after 15 min and passed the entire small intestine within 1 h after its administration, demonstrating that 4-kDa FITC dextran measured in plasma 1 h after its oral application is a marker of small intestinal permeability. Dulbeccos Phosphate-Buffered Saline (DPBS) was purchased from Welgene (Gyeongsan, Korea). Walkenhorst, S.; Olivier, R. (2003). Lpez Martnez, M. C.; Daz Baos, F. G.; Ortega Retuerta, A.; Garca de , J. Radiofrequency with microneedling. 20K groups (n=3, *: p<0.05, **: p<0.01). volume24, Articlenumber:22 (2020) Materiaux 1-5. The same way, many of these authors emphasize the non-linear or hyperbranched of dextran molecular weight range from 9 to 2000kDa [3,5,8,16, 37,38]. 2015 Mar 17;6:155. doi: 10.3389/fmicb.2015.00155. The term f/f0 is sometimes denoted as P, Perrin constant. hydrodynamic WebDextran produced from Weissella cibaria JAG8 was purified and characterized. The Dextrans intrinsic viscosity is determined as a function of the molecular weight in water and NaOH 0.5 M see reference [1]. The skin culture medium was purchased from Biopredic International (Saint-Grgoire, France). European Biophysical Journal, 36, 85-94. Hydrodynamic The hydrodynamic radius R h, defined as the radius of a hard sphere that diffuses at the same rate as the solute, takes these effects into account. WebFluorescent and biotinylated dextrans are routinely employed to trace neuronal projections. J Pharm Sci. Hydrodynamic properties, such as the intrinsic viscosity, [], and equilibrium solution properties such as the hydrodynamic radius RH can be combined to construct dimensionless quantities that are universal since they are of being independent of the macromolecular particle size, while they depend more or less sensitively on its shape [51].
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